Lasseter lives in Glen Ellen, California with his wife Nancy, a graduate of Carnegie Mellon University whom he met at a computer graphics conference. He has five sons, their ages ranging from 10 to 29. The family has a Dachshund named Frank and Lasseter's father, Paul, also lives on the property. The Lasseters own vineyards located in Glen Ellen, where they make and sell their own label of wine. Nancy retired to take care of their children and work on their vineyard. He is an avid NASCAR fan and loves to attend the races at Infineon Raceway in Sonoma, California.

On May 2, 2009, Lasseter received an Honorary Doctorate degree from Pepperdine University. He gave a commencement address where he encouraged the graduating class of more than 500 students never to let anyone kill their dreams.